Dissociation of Glyceraldehyde-3-Phosphate Dehydrogenase with Sodium Dodecyl Sulfate
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منابع مشابه
Purification and Characterization of Glycerol-3-phosphate Dehydrogenase (Flavin-linked) from Rat Liver
We have purified the membrane-intrinsic glycerol3-phosphate dehydrogenase from both normal and hyperthyroid rat liver mitochondria by extraction with Triton X-100, hydrophobic affinity chromatography, ion exchange chromatography, gel filtration, and FAD-linked Sepharose 4B affinity chromatography. The yields in both cases were over 20‘70, and purification ranged from 800to 650-fold in mitocho...
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The molecular mechanisms involved in the degradation of individual cellular proteins are probably unique and characteristic. We have investigated in rat liver the degradation of glyceraldehyde-3-phosphate dehydrogenase, an abundant cytosolic enzyme of the glycolytic pathway. Immunoblot analysis of isolated liver lysosomes from rats treated with lysosomal inhibitors show that this protein is deg...
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Glyceraldehyde 3-phosphate dehydrogenase is one of the major protein components associated with the erythrocyte membrane. This has been shown by activity studies of specific membrane extracts and by the specificity of iodoacetate inactivation and labeling of the enzyme. Studies on the association of the enzyme with the membrane are complicated by the tendency of the ghosts to seal under certain...
متن کاملPurification and characterization of glyceraldehyde-3-phosphate dehydrogenase from European pilchard Sardina pilchardus.
The NAD+-dependent cytosolic glyceralehyde-3-phosphate dehydrogenase (GAPDH; EC 1.2.1.12) was purified from the skeletal muscle of European pilchard Sardina pilchardus and its physicochemical and kinetic properties were investigated. The purification method consisted of two steps, ammonium sulfate fractionation followed by Blue Sepharose CL-6B chromatography, resulting in an approximately 78-fo...
متن کاملStudy of a kindred with hereditary spherocytosis and glyceraldehyde-3-phosphate dehydrogenase deficiency.
A patient with hereditary spherocytosis (HS) was found to have glyceraldehyde-3-phosphate dehydrogenase (G3PD) deficiency by electrophoresis of the isolated red cell membranes on polyacrylamide gels with sodium dodecyl sulfate (PAGE SDS) as demonstrated by a diminished band 6 (G3PD) and confirmed by specific enzyme assay. Thirteen members of his family were studied: four were normal, two had HS...
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تاریخ انتشار 2017